Microbial Glass Fermente
BFS glass fermenter refers to a jacketed fermenter with 2 L, 5 L, 10 L or other customized volumes. It can be used for the cultivation of escherichia coli, yeast and fungus. The fermenter has more than ten functional modules, including air intake/exhaust, liquid replenishing, temperature control, sampling, various stirring systems and stirring paddles, feeding bottles, heat exchangers and baffles. These modules can be added or removed as needed by customers.
Design features
1. Each functional module is easy to operate and clean
2. Function modularization: various functional modules are provided based on the requirements of customers
3. Flexibility: the system configuration can be adjusted according to the customers' process requirements
4. It is designed for laboratory-level or small experiment-level microbial fermentation
5. Two-path optional gases are provided to meet the needs of microbial fermentation and cultivation
6. Humanized airflow control and selection are supported to meet your particular needs
7. The control interface is concise and readily understood
8. The built-in associated interface can help the control of dissolved oxygen
9. Adjustable PID value is adopted to control the pH and dissolved oxygen amount
Technical parameters
1. The fermenter has 2 L, 5 L, 10 L or other customized volumes, with a loading factor of 70%
2. Double-layer borosilicate glass tank body is used with a height-diameter ratio of 1: 2-3. The cover is made of stainless steel, and the electropolished inner surface shows a Ra＜0.8 μm
3. Top stirring (mechanical/magnetic) is adopted. Two layers of straight-blade disc turbine paddles, one layer of inclined-blade disk turbine paddles, and one layer of mechanical defoaming paddles are used, and removable striker plate is installed inside the fermenter
4. The controller adopts a 15-inch touch screen and Siemens S7 series PLC as the control core, supporting the set and control of pH, DO, temperature, stirring form, foam, feeding and aeration of the fermenter. All process parameters are displayed on the touch screen, and the set values and actual values can be shown in the form of numbers, line graphs and column diagrams
5. The controller can grant different users with different authorities through various passwords to ensure the safety of the cultivation process to the maximum extent
6. The modular design makes it easy to add or remove modules to suit different applications and change the process control strategies
7. PID control: PID control (including manual PID control and adaptive PID control) is adopted for all the process parameters to ensure the accuracy and precision of the experiment
8. Temperature control: the temperature is adjusted by means of the heating blanket and built-in cooling water coil with a temperature range of 5 ℃-60 ℃ and accuracy of ± 0.2 ℃
9. Stirring speed control: the rotation speed range is 10-1,000 rpm, with an accuracy of ± 2 rpm. It can be controlled in association with other parameters. The stirring motor is equipped with an encoder for feedback loop to ensure accurate control of the stirring speed
10. pH control: equipped with long-life and high-precision imported pH electrodes; control range: 0-14 pH, control accuracy: ± 0.01 pH; The pH is controlled by the automatic addition of alkali by peristaltic pump
11. DO control: equipped with long life and low-drift imported DO electrodes; control range: 0-120%, control accuracy: ± 0.1%; The cascade control of rotation speed, aeration volume, or volume of the pure oxygen and nitrogen is adopted for the dissolved oxygen
12. Foam control: foam electrode shows a high conductivity > 26 μS-100% and a low conductivity＞200 μS-100%. The foam is controlled by liquid level sensing of the foam probe and the automatic addition of the defoamer
13. Aerating system: equipped with air and oxygen paths with imported rotator gas flowmeters for independent measurement. Subsequently, other gas paths can be increased according to the process requirements, and each path of gas can be separately equipped with a mass flowmeter and easily changed from submerged aeration to surface aeration. Submerged aeration includes interchangeable fine-bubble aeration and coarse-bubble aeration
14. Peristaltic pump feeding system: the tank body is equipped with 3 constant-speed peristaltic pumps and a variable-speed peristaltic pump for feeding of alkali liquor, defoamer and materials. Continuous or intermittent operation is controlled by the software as desired
15. A tail gas condenser is set at the tail gas outlet to reduce evaporation of the culture medium
16. The process software can collect on-line/off-line data, export data in the format of EXEL and BMP files. The data can be shown in the form of in scatter diagrams, continuous line diagrams, sketch maps and sheets. It supports the integration of current and past data, and data of different batches. It can show the measurement data in sheets and scatter diagrams. In addition, the preset graphic settings can be stored
17. Outline dimension: glass tank of 250*250*700 mm and control cabinet of 600*400*930 mm.
